DOT · Regulated Modes
Every DOT Mode. One Program.
The U.S. Department of Transportation regulates six operating administrations - each with its own testing rule, random rate, and pool requirement. 365 EasyScreen handles all of them on a single platform.
Modalities & Testing Requirements
Each agency governs its own safety-sensitive workforce under Part 40 protocols. Populations, random rates, and recordkeeping differ - chain of custody does not.
FMCSA
49 CFR Part 382
Commercial Motor Carriers
CDL drivers operating commercial motor vehicles. Pre-employment, random, post-accident, return-to-duty, and follow-up testing. Trucking is the largest DOT modality.
Random rate
50% drug · 10% alcohol
Covered
CDL drivers
Multi-mode employers, one system of record
Operate under more than one DOT agency? Stop juggling pools, MROs, and audit trails. Here’s what consolidation looks like in practice.
The legacy problem
Trucks under FMCSA, a hazmat pipeline under PHMSA, ferries under USCG - each agency maintains its own pool, MRO, and audit trail. We keep those pools separate and manage every agency from one platform.
One source-verified record
Testing, results, and randoms are managed in one system and surfaced per-agency for audits - tied to the same driver, mariner, or technician file.
Under-50 consortium pooling
Consortium access is available for all modes with fewer than 50 covered employees. Setup and handling vary by agency, but every mode can join our shared DOT consortium for random pooling.
Supporting DOT-Regulated Employers
Federal regulations can be complex. We help employers navigate the requirements, understand available testing options, and connect with trusted regulatory resources so they can make informed decisions for their workplace testing program.
THE KEYSTONE REGULATION
49 CFR Part 40
Establishes the procedures for workplace drug and alcohol testing across all DOT agencies.
OPERATING ADMINISTRATIONS
All DOT-regulated testing programs should be administered in accordance with applicable federal regulations and agency guidance. Employers remain responsible for their compliance programs.
Questions? We have answers.
Answers to common questions about DOT drug and alcohol testing programs across all federally regulated transportation industries.
A DOT drug and alcohol testing program is required for employees who perform safety-sensitive duties under DOT regulations. Depending on your operating administration, your program may include pre-employment, random, reasonable suspicion, post-accident, return-to-duty, and follow-up testing.
The U.S. Department of Transportation oversees several operating administrations, each with its own regulations for safety-sensitive employees. These include the FMCSA, FAA, FRA, FTA, PHMSA, and USCG. If you're unsure which regulations apply to your workforce, our team can help point you to the appropriate agency resources.
All DOT agencies follow the federal testing procedures established under 49 CFR Part 40, but each operating administration has additional agency-specific regulations and requirements. Employers should refer to their governing agency for program-specific guidance.
Yes. We support employers operating under multiple DOT agencies and can coordinate testing services through a nationwide collection network while helping you navigate the different testing requirements for each mode.
Yes. In addition to DOT-regulated testing, we provide non-DOT drug screening, occupational health services, and background screening for employers across a wide range of industries. Many employers manage both regulated and non-regulated employees through a single program.
We encourage employers to use official federal resources when developing or maintaining their workplace testing programs. Helpful resources include 49 CFR Part 40, the DOT Office of Drug and Alcohol Policy & Compliance (ODAPC), and your operating administration's website.

