DOT · Regulated Modes

Every DOT Mode. One Program.

The U.S. Department of Transportation regulates six operating administrations - each with its own testing rule, random rate, and pool requirement. 365 EasyScreen handles all of them on a single platform.

The Six Operating Administrations

Modalities & Testing Requirements

Each agency governs its own safety-sensitive workforce under Part 40 protocols. Populations, random rates, and recordkeeping differ - chain of custody does not.

FMCSA

49 CFR Part 382

Commercial Motor Carriers

CDL drivers operating commercial motor vehicles. Pre-employment, random, post-accident, return-to-duty, and follow-up testing. Trucking is the largest DOT modality.

Random rate

50% drug · 10% alcohol

Covered

CDL drivers

Trucking consortium →
Why One Program

Multi-mode employers, one system of record

Operate under more than one DOT agency? Stop juggling pools, MROs, and audit trails. Here’s what consolidation looks like in practice.

The legacy problem

Trucks under FMCSA, a hazmat pipeline under PHMSA, ferries under USCG - each agency maintains its own pool, MRO, and audit trail. We keep those pools separate and manage every agency from one platform.

One source-verified record

Testing, results, and randoms are managed in one system and surfaced per-agency for audits - tied to the same driver, mariner, or technician file.

Under-50 consortium pooling

Consortium access is available for all modes with fewer than 50 covered employees. Setup and handling vary by agency, but every mode can join our shared DOT consortium for random pooling.

Talk to a DOT Specialist
DOT Compliance Resources

Supporting DOT-Regulated Employers

Federal regulations can be complex. We help employers navigate the requirements, understand available testing options, and connect with trusted regulatory resources so they can make informed decisions for their workplace testing program.

THE KEYSTONE REGULATION

49 CFR Part 40

Establishes the procedures for workplace drug and alcohol testing across all DOT agencies.

View Regulations
DOT Program FAQs

Questions? We have answers.

Answers to common questions about DOT drug and alcohol testing programs across all federally regulated transportation industries.

  • A DOT drug and alcohol testing program is required for employees who perform safety-sensitive duties under DOT regulations. Depending on your operating administration, your program may include pre-employment, random, reasonable suspicion, post-accident, return-to-duty, and follow-up testing.

  • The U.S. Department of Transportation oversees several operating administrations, each with its own regulations for safety-sensitive employees. These include the FMCSA, FAA, FRA, FTA, PHMSA, and USCG. If you're unsure which regulations apply to your workforce, our team can help point you to the appropriate agency resources.

  • All DOT agencies follow the federal testing procedures established under 49 CFR Part 40, but each operating administration has additional agency-specific regulations and requirements. Employers should refer to their governing agency for program-specific guidance.

  • Yes. We support employers operating under multiple DOT agencies and can coordinate testing services through a nationwide collection network while helping you navigate the different testing requirements for each mode.

  • Yes. In addition to DOT-regulated testing, we provide non-DOT drug screening, occupational health services, and background screening for employers across a wide range of industries. Many employers manage both regulated and non-regulated employees through a single program.

  • We encourage employers to use official federal resources when developing or maintaining their workplace testing programs. Helpful resources include 49 CFR Part 40, the DOT Office of Drug and Alcohol Policy & Compliance (ODAPC), and your operating administration's website.